Improved Detection of Viable Escherichia coli O157:H7 in Milk by Using Reverse Transcriptase-PCR
نویسندگان
چکیده
منابع مشابه
Detection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
متن کاملApplication of Reverse Transcriptase –PCR (RT-PCR) for rapid detection of viable Escherichia coli in drinking water samples
BACKGROUND Polymerase chain reaction (PCR) is preferred to other methods for detecting Escherichia coli (E. coli) in water in terms of speed, accuracy and efficiency. False positive result is considered as the major disadvantages of PCR. For this reason, reverse transcriptase-polymerase chain reaction (RT-PCR) can be used to solve this problem. The aim of present study was to determine the effi...
متن کاملDirect detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.
A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other ...
متن کاملPCR-ELISA detection of Escherichia coli in milk.
AIMS The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk. METHODS AND RESULTS Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA. CONCLUSIONS It was found that 5 E. coli co...
متن کاملDetection of Colicin genes by PCR in Escherichia coli isolated from cattle in Shiraz-Iran
A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Korean Journal for Food Science of Animal Resources
سال: 2011
ISSN: 1225-8563
DOI: 10.5851/kosfa.2011.31.2.158